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Three hemoglobin components may be separated from hemolysates of normal adult red blood cells by starch block electrophoresis: the main component, A₁, moves at a position intermediate between the most slowly moving component, A₂, and the most rapidly moving component, A₃. By chromatography, eight hemoglobin components may be detected: five (A_ (Ia), A_ (Ib), A_ (Ic), A_ (Id) and A_ (Ie) ) move down the column more rapidly than the main component (An) and two move more slowly (Ania and Amb). Non-heme containing proteins are also present. The present investigation has determined the correspondence the components as isolated by the two methods: AI contains AI (c), A_ (Id) and A_ (Ie), and A_ (II) ; A₂ contains _ (IIIb) and non-heme protein (s) ; A₃ contains A_ (Ia), A (Ib) and non-heme protein (s).
Schnek et al. (Wed,) studied this question.