Pre-treatment with sacubitril and valsartan significantly prevented antioxidant enzyme degradation, reduced cardiac biomarkers, and decreased infarction area in rats with isoproterenol-induced MI.
Does sacubitril and valsartan pre-treatment prevent myocardial damage in an isoproterenol-induced myocardial infarction rat model?
Sacubitril and valsartan protect against isoproterenol-induced myocardial infarction in rats by reducing oxidative stress and myocardial damage.
BACKGROUND: Sacubitril (SAC), a neprilysin inhibitor prevent degradation of neprilysin and activate cGMP signaling pathways leading to rise in blood volume concurrent to blood pressure by means of vasoactive peptides, adrenomedullin, and bradykinin. OBJECTIVE: The aim of this study was to evaluate the anti-ischemic effects of SAC through inhibiting neprilysin in isoproterenol (ISO) induced myocardial infarction (MI) in Wistar albino rats. ISO (85 mg/kg) was injected subcutaneously at the end of 14 days pre-treatment with SAC and valsartan (VAL). RESULT: Biochemical investigation revealed that SAC along with VAL significantly prevented the antioxidant enzymes (SOD, Catalase, GR, GPx, GST, and GSH) degradation and malondialdehyde (MDA) induced by ISO intoxication in Wistar rats. Along with this, cardiac biomarkers (LDH, CK-MB, ALT, AST, and ALP) were also significantly ameliorated by SACand VAL in ISO-treated rats. Concurrently, decreased infarction area (IA)and marked reduction in myofibril damage by SACand VAL further supported its protective benefits in MI. CONCLUSION: Taken together, the results suggest that inhibition of enzyme neprilysin alleviated the ISO induces myocardial damage mediated by its strong antioxidant potential.
Imran et al. (Thu,) conducted a other in Isoproterenol-induced myocardial infarction. Sacubitril and valsartan vs. Isoproterenol alone was evaluated on Antioxidant enzymes, malondialdehyde, cardiac biomarkers, infarction area, and myofibril damage. Pre-treatment with sacubitril and valsartan significantly prevented antioxidant enzyme degradation, reduced cardiac biomarkers, and decreased infarction area in rats with isoproterenol-induced MI.