Adoptively transferred regulatory T cells greatly reduced left-ventricular fibrosis, inflammatory cell numbers, and TGF-β1 system activity in mice with aortic constriction-induced hypertension.
Does adoptive transfer of CD4+CD25+Foxp3+ regulatory T cells reduce left ventricular fibrosis in mice with aortic constriction-induced hypertension?
Adoptive transfer of regulatory T cells attenuates cardiac fibrosis and inflammation in a mouse model of hypertensive heart disease.
BACKGROUND: CD4⁺CD25⁺Foxp3⁺ regulatory T cells (Tregs) are potent inhibitors of inflammation and autoimmune diseases. Because inflammation has been associated with development of cardiac fibrosis in experimental hypertension, here we investigated whether adoptively transferred Tregs would inhibit development of cardiac fibrosis initiated by elevating blood pressure. METHODS: Cardiac fibrosis was induced in mice by constricting the aorta between the two carotid arteries. Immediately after the operation mice received either vehicle or purified, cultured Tregs (1.5 × 10⁶). Fourteen days later we assessed effects on developing left ventricular fibrosis, blood pressure, inflammation, myofibroblasts and the transforming growth factor-beta1 (TGF-β1) system. RESULTS: Fourteen days after aortic constriction, marked left-ventricular fibrosis was apparent and this was greatly reduced in mice receiving adoptively transferred Tregs. This reduction in fibrosis was associated with attenuated inflammatory cell numbers, reduced interstitial myofibroblast numbers and attenuated activity of the TGF-β1 system, indicated by reductions in the expression of TGF-β1 and its receptors activin-like kinase-5 and type II TGF-β receptor. Adoptively transferred Tregs did not affect blood pressure and exerted only a small effect on left-ventricular hypertrophy. CONCLUSIONS: These data indicate that Tregs attenuate cardiac fibrosis associated with hypertensive heart disease by suppressing inflammation.
Kanellakis et al. (Sat,) conducted a other in Cardiac fibrosis in experimental hypertension. Adoptively transferred CD4+CD25+Foxp3+ regulatory T cells (Tregs) vs. Vehicle was evaluated on Left ventricular fibrosis, blood pressure, inflammation, myofibroblasts and the transforming growth factor-beta1 (TGF-β1) system. Adoptively transferred regulatory T cells greatly reduced left-ventricular fibrosis, inflammatory cell numbers, and TGF-β1 system activity in mice with aortic constriction-induced hypertension.