Does an optimized real-time RT-PCR assay improve sensitivity and reproducibility for detecting enterovirus RNA compared to an earlier assay?
An optimized real-time RT-PCR assay improves the detection of enterovirus RNA with higher sensitivity and reduced hands-on time.
We describe an improvement of an earlier reported real-time RT-PCR assay for the detection of enterovirus RNA, based on the 5' exonuclease digestion of a dual-labeled fluorogenic probe by Taq DNA polymerase. A different extraction method, real-time RT-PCR instrument and primer set were evaluated. Our data show that the optimized assay yields a higher sensitivity and reproducibility and resulted in a significant reduced hands-on time per sample.
Piqueur et al. (Tue,) studied this question.