Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells.

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Abstract

The DNA coding sequence for the hygromycin B phosphotransferase gene was placed under the control of the regulatory sequences of a cloned long terminal repeat of Moloney sarcoma virus. This construction allowed direct selection for hygromycin B resistance after transfection of eucaryotic cell lines not naturally resistant to this antibiotic, thus providing another dominant marker for DNA transfer in eucaryotic cells.

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Karen Blöchlinger

Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa

Heidi Diggelmann

University of Bern

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Molecular and Cellular Biology

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Swiss Group For Clinical Cancer Research

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Blöchlinger et al. (Sat,) studied this question.

synapsesocial.com/papers/6a2234d7e8ef4064f24ecf82 — DOI: https://doi.org/10.1128/mcb.4.12.2929

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Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells.

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