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Development of a sensitive assay for SERCA activity using FRET detection of ADP | Synapse

January 1, 2014

Development of a sensitive assay for SERCA activity using FRET detection of ADP

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Abstract

A SERCA activity assay which detects ADP productionviatime-resolved FRET is reported, improving sensitivity 50-fold and sample volume 1000-fold.

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Cite This Study

Meng et al. (Wed,) studied this question.

synapsesocial.com/papers/6a236f0f3bc5b5ee874ca299 https://doi.org/https://doi.org/10.1039/c3ay41808a

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3Comparison of SERCA1 and SERCA2a expressed in COS-1 cells and cardiac myocytes1999 · 64 citations
4NADH-coupled microplate photometric assay for kinetic studies of ATP-hydrolyzing enzymes with low and high specific activities2003 · 188 citations
5Alterations in the structure of the ribose moiety of ATP reduce its effectiveness as a substrate for the sarcoplasmic reticulum ATPase.1983 · 51 citations