Key points are not available for this paper at this time.
Making up for lost time: The one-pot synthesis of crambin (see structure) with only a single final purification step gave the target protein of exceptional purity in only two days with an overall yield of ≈40 %. Three unprotected peptide segments were linked by native chemical ligation, and the polypeptide chain assumed its 3D structure without intermediate purification steps. Supporting information for this article is available on the WWW under http: //www. wiley-vch. de/contents/jc₂002/2004/z53540ₛ. pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Bang et al. (Wed,) studied this question.