Recombinant simian rotaviruses carrying heterologous VP4 and VP7 genes from clinical isolates were successfully generated and can be used for antigenic characterization and vaccine production.
A reverse genetics system can successfully generate chimeric rotaviruses incorporating genes from clinical isolates, providing a valuable tool for vaccine development and antigenic characterization.
Although vaccines have reduced global RV-associated hospitalization and mortality over the past decade, the multisegmented genome of RVs allows reassortment of VP4 and VP7 genes from different RV species and strains. The evolutionary dynamics of novel RV genotypes and their constellations have led to great genomic and antigenic diversity. The reverse genetics system is a powerful tool for manipulating RV genes, thereby controlling viral antigenicity, growth capacity, and pathogenicity. Here, we generated recombinant simian RVs (strain SA11) carrying heterologous VP4 and VP7 genes cloned from clinical isolates and showed that VP4- or VP7-substituted chimeric viruses can be used for antigenic characterization of RV outer capsid proteins and as improved seed viruses for vaccine production.
Kanai et al. (Thu,) conducted a other in Rotavirus infection. Recombinant simian RVs (strain SA11) carrying heterologous VP4 and VP7 genes was evaluated on Generation and characterization of recombinant simian RVs. Recombinant simian rotaviruses carrying heterologous VP4 and VP7 genes from clinical isolates were successfully generated and can be used for antigenic characterization and vaccine production.
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