Heterotopic transplantation of abdominal aortic constriction in rats resulted in a 54% decrease in cardiomyocyte cross-sectional area compared to AAC alone, with significant changes in miR-23a and miR-29a.
Changes in miR-23a and miR-29a expression during cardiac hypertrophy and reverse remodeling suggest they may be potential therapeutic targets for heart remodeling.
BACKGROUND: MicroRNAs (miRNAs) may serve as potential diagnostic biomarkers and therapeutic targets in cardiovascular research. However, the association between miRNAs and heart remodeling/reverse remodeling has not been evaluated. METHODS: Lewis rats were divided into three groups: control animals, animals subjected to abdominal aortic constriction (AAC) and those with heterotopic transplantation of abdominal aortic constriction (AAC-HT), respectively. The cardiomyocyte cross-sectional area and changes in the heart cavity were determined. miRNA microarray was used to search for changes in miRNAs during hypertrophy and in the unloading heart, which was also verified using real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). RESULTS: The AAC-HT group exhibited a 54% decrease in the cardiomyocyte cross-sectional area compared to the AAC group. In the two test groups, miRNA microarrays revealed changes in 293 miRNAs, among which 40 miRNAs changed >2-fold. Some major changes were also confirmed using qRT-PCR primers. The results indicated that changes in miR-23a and miR-29a were most significant, thus suggesting that these miRNAs may play important roles in heart remodeling and reverse remodeling. CONCLUSIONS: The changes observed in miRNA expression during hypertrophy and reverse remodeling may indicate possibly meaningful targets for regulating the remodeling or reverse remodeling of the heart.
Wang et al. (Wed,) conducted a other in Heart remodeling and reverse remodeling. Abdominal aortic constriction (AAC) and heterotopic transplantation (AAC-HT) vs. Control animals and AAC alone was evaluated on Cardiomyocyte cross-sectional area and miRNA expression changes. Heterotopic transplantation of abdominal aortic constriction in rats resulted in a 54% decrease in cardiomyocyte cross-sectional area compared to AAC alone, with significant changes in miR-23a and miR-29a.
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