Figure 3 from Generation, Transcriptomic States, and Clinical Relevance of CX3CR1+ CD8 T Cells in Melanoma

The differentiation of CX3CR1− to CX3CR1+ CD8+ T cells could occur in the periphery without migration to secondary lymphoid organs. A, Experimental protocol. B, Frequency of endogenous CD90.1− CD8+ T cells and infused Pmel-1 CD90.1+ CD8+ T cells in the spleen was analyzed after treatment with FTY720 or control vehicle. Data right show the frequency of endogenous CD90.1− CD8+ T cells and infused Pmel-1 CD90.1+ CD8+ T cells. C, Representative contour plots showing CD27/CX3CR1 expression of CD8 and CD90.1-gated cells in the spleen (top) and the tumor (bottom). Numbers, percentage of the CX3CR1+ subset. Data right show the frequency of CX3CR1+ CD8+ T cells obtained from seven mice in each group. P values by two-tailed unpaired t test. NS, not significant. Mean (±SEM).