Figure 3 from Generation, Transcriptomic States, and Clinical Relevance of CX3CR1⁺ CD8 T Cells in Melanoma

The differentiation of CX3CR1⁻ to CX3CR1⁺ CD8⁺ T cells could occur in the periphery without migration to secondary lymphoid organs. A, Experimental protocol. B, Frequency of endogenous CD90.1⁻ CD8⁺ T cells and infused Pmel-1 CD90.1⁺ CD8⁺ T cells in the spleen was analyzed after treatment with FTY720 or control vehicle. Data right show the frequency of endogenous CD90.1⁻ CD8⁺ T cells and infused Pmel-1 CD90.1⁺ CD8⁺ T cells. C, Representative contour plots showing CD27/CX3CR1 expression of CD8 and CD90.1-gated cells in the spleen (top) and the tumor (bottom). Numbers, percentage of the CX3CR1⁺ subset. Data right show the frequency of CX3CR1⁺ CD8⁺ T cells obtained from seven mice in each group. P values by two-tailed unpaired t test. NS, not significant. Mean (±SEM).