FIGURE 5 from Exhausted Tumor-infiltrating CD39+CD103+ CD8+ T Cells Unveil Potential for Increased Survival in Human Pancreatic Cancer

Characterization of CD39+CD103+ CD8+ T cells. A, Representative flow cytometry histograms showing the data in B. B, Proportion of CD39− CD103+ (SP CD103), CD39+ CD103+ (DP), and CD39− CD103− (DN) CD8+ T cells expressing PD-1 (n = 36), TIM-3 (n = 36), PD-1-TIM-3 (n = 36), Ki67 (n = 6), and TCF1 (n = 6) in central and peripheral tissues. C, Representative flow cytometry histograms showing the data in D. D, Proportion of DP and DN CD8+ T cells expressing CCR5, CXCR6, CXCR3, CXCR4, and CXCR5 in central and peripheral tissues of 6 patients. E, Representative zebra plots showing the gating strategy for DP CD8+ T cells with high expression of CD39 (CD103+ CD39high) and low expression of CD39 (CD103+ CD39+). F, Proportion of CD103+ CD39+ and CD103+ CD39high expressing PD-1-TIM-3, CCR5, CXCR3, CXCR6, Ki67, and TCF1. F, Left, Representative flow cytometry histograms. Right, Proportion of granzyme B, perforin, and granzyme B-perforin on CD39+ CD103+ DP and CD39− CD103− DN CD8+ T cells from central and peripheral tumor tissues from 7 patients. D and F, Friedman test followed by Dunn test was used to evaluate significant difference between groups. *, P P P E, Wilcoxon matched pairs signed-rank test was used to detect statistically significant differences. *, P P P