FIGURE 5 from Exhausted Tumor-infiltrating CD39⁺CD103⁺ CD8⁺ T Cells Unveil Potential for Increased Survival in Human Pancreatic Cancer

Characterization of CD39⁺CD103⁺ CD8⁺ T cells. A, Representative flow cytometry histograms showing the data in B. B, Proportion of CD39⁻ CD103⁺ (SP CD103), CD39⁺ CD103⁺ (DP), and CD39⁻ CD103⁻ (DN) CD8⁺ T cells expressing PD-1 (n = 36), TIM-3 (n = 36), PD-1-TIM-3 (n = 36), Ki67 (n = 6), and TCF1 (n = 6) in central and peripheral tissues. C, Representative flow cytometry histograms showing the data in D. D, Proportion of DP and DN CD8⁺ T cells expressing CCR5, CXCR6, CXCR3, CXCR4, and CXCR5 in central and peripheral tissues of 6 patients. E, Representative zebra plots showing the gating strategy for DP CD8⁺ T cells with high expression of CD39 (CD103⁺ CD39high) and low expression of CD39 (CD103⁺ CD39⁺). F, Proportion of CD103⁺ CD39⁺ and CD103⁺ CD39high expressing PD-1-TIM-3, CCR5, CXCR3, CXCR6, Ki67, and TCF1. F, Left, Representative flow cytometry histograms. Right, Proportion of granzyme B, perforin, and granzyme B-perforin on CD39⁺ CD103⁺ DP and CD39⁻ CD103⁻ DN CD8⁺ T cells from central and peripheral tumor tissues from 7 patients. D and F, Friedman test followed by Dunn test was used to evaluate significant difference between groups. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant. E, Wilcoxon matched pairs signed-rank test was used to detect statistically significant differences. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.