RAA-CRISPR-HCR cascade amplification for ultrasensitive visual detection of African Swine Fever Virus DNA

Puntos clave

• The aim is to develop a highly sensitive method for detecting African Swine Fever Virus DNA using an amplification system.
• Developed an RAA-CRISPR-HCR cascade amplification system.
• Utilized colorimetric techniques for detection.
• Tested sensitivity to low concentrations of ASFV DNA.
• Achieved a detection limit of 1 copy of ASFV DNA.
• Produced a strong colorimetric response at low DNA concentrations.