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February 2, 2026Chemical Communications1 citations

RAA-CRISPR-HCR cascade amplification for ultrasensitive visual detection of African Swine Fever Virus DNA

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HWHongyu WuJinan UniversitySLShuhan LinNanjing University of Posts and TelecommunicationsXZXiangcun ZuoNanjing University of Posts and Telecommunications

Key Points

  • The aim is to develop a highly sensitive method for detecting African Swine Fever Virus DNA using an amplification system.
  • Developed an RAA-CRISPR-HCR cascade amplification system.
  • Utilized colorimetric techniques for detection.
  • Tested sensitivity to low concentrations of ASFV DNA.
  • Achieved a detection limit of 1 copy of ASFV DNA.
  • Produced a strong colorimetric response at low DNA concentrations.

Abstract

An RAA-CRISPR-HCR (RCH) cascade amplification system was developed, which generates an ultrasensitive colorimetric response to low concentrations of African Swine Fever Virus (ASFV) DNA with a detection limit of 1...

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Cite This Study

Wu et al. (2026) studied this question.

synapsesocial.com/papers/6980fe00c1c9540dea80fbcchttps://doi.org/10.1039/d5cc06815k
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