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The use of DAPI for identifying and counting aquatic microflora1 | Synapse

September 1, 1980Open Access

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The use of DAPI for identifying and counting aquatic microflora1

KPKaren G. PorterPiedmont Athens Regional YFYvette S. FeigUniversity of Georgia

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Resumen

A highly specific and sensitive fluorescing DNA stain, 4′6‐diamidino‐2‐phenylindole (DAPI) was compared with acridine orange (AO) for counting aquatic microflora. Use of DAPI improved visualization and counting of <1‐ µ m bacteria and blue‐green algae in seston‐rich samples and extended sample storage to at least 24 weeks.

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Cite This Study

Porter et al. (Mon,) studied this question.

synapsesocial.com/papers/69da9b00a6045d71bfa3d311 https://doi.org/https://doi.org/10.4319/lo.1980.25.5.0943

Also Consider

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1Use of nuclepore filters for counting bacteria by fluorescence microscopy1977 · 4,281 citations
2Direct counts of aquatic bacteria by a modified epifluorescence technique11975 · 326 citations
3Acridine Orange-Epifluorescence Technique for Counting Bacteria in Natural Waters1973 · 203 citations
4Fluxes, dynamics and chemistry of particulates in the ocean1977 · 61 citations
5Fluorescence Properties of the Monoaminoacridines and Some 2-Aminoacridine-Derivatives1973 · 13 citations