The use of DAPI for identifying and counting aquatic microflora1

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Abstract

A highly specific and sensitive fluorescing DNA stain, 4′6‐diamidino‐2‐phenylindole (DAPI) was compared with acridine orange (AO) for counting aquatic microflora. Use of DAPI improved visualization and counting of <1‐ µ m bacteria and blue‐green algae in seston‐rich samples and extended sample storage to at least 24 weeks.

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Karen G. Porter

Piedmont Athens Regional

Yvette S. Feig

University of Georgia

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Limnology and Oceanography

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University of Georgia

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Porter et al. (Mon,) studied this question.

synapsesocial.com/papers/69da9b00a6045d71bfa3d311 — DOI: https://doi.org/10.4319/lo.1980.25.5.0943

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Native Aquatic Bacteria: Enumeration, Activity, and Ecology· 1979 · 126 citations
Direct counts of aquatic bacteria by a modified epifluorescence technique1· 1975 · 326 citations
A NEW METHOD FOR FLUORESCENCE STAINING OF BACTERIAL POPULATIONS ON MEMBRANE FILTERS· 1974 · 212 citations

Also consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

Use of nuclepore filters for counting bacteria by fluorescence microscopy· 1977 · 4,277 citations
Acridine Orange-Epifluorescence Technique for Counting Bacteria in Natural Waters· 1973 · 203 citations
Native Aquatic Bacteria: Enumeration, Activity, and Ecology· 1979 · 126 citations
Direct counts of aquatic bacteria by a modified epifluorescence technique1· 1975 · 326 citations
A NEW METHOD FOR FLUORESCENCE STAINING OF BACTERIAL POPULATIONS ON MEMBRANE FILTERS· 1974 · 212 citations

The use of DAPI for identifying and counting aquatic microflora1

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Abstract

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References and Citations

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Discussion

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