This study is the first report of the metal-dependent stimulation of compromised activities in a DNA glycosylase variant. The MD simulations suggest that Mg2+ stabilizes the surface loop dynamics in the T103A variant, with the effects of the T to A substitution and Mg2+ presence being transmitted over a distance greater than 26 Å to the NEIL1 active site.
Zuckerman et al. (Mon,) studied this question.