Hyperinsulinemia and regional myocardial ischemia additively increased myocardial glucose uptake by 50% and augmented sarcolemmal GLUT1 and GLUT4 contents by 20% and 60%, respectively (P<0.05).
Does hyperinsulinemia combined with ischemia increase myocardial GLUT1 and GLUT4 translocation in vivo in fasted anesthetized dogs?
Insulin and ischemia have additive effects to increase in vivo myocardial glucose utilization and augment GLUT1 and GLUT4 translocation.
valor p: p=<0.05
BACKGROUND: Myocardial ischemia increases glucose uptake through the translocation of GLUT1 and GLUT4 from an intracellular compartment to the sarcolemma. The present study was performed to determine whether hyperinsulinemia causes translocation of myocardial GLUT1 as well as GLUT4 in vivo and whether there are additive effects of insulin and ischemia on GLUT1 and GLUT4 translocation. METHODS ADN RESULTS: Myocardial glucose uptake and transporter distribution were assessed by arteriovenous measurements, cell fractionation, and immunofluorescence. In fasted anesthetized dogs, hyperinsulinemia increased myocardial glucose extraction 3-fold (P<0.01) and the sarcolemmal content of GLUT4 by 90% and GLUT1 by 50% (P<0.05 for both) compared with saline infusion. In subsequent experiments, glucose uptake and transporter distribution were determined in ischemic and nonischemic regions of hearts from hyperinsulinemic animals during regional myocardial ischemia. Glucose uptake was 50% greater in the ischemic region (P<0.05). This was associated with a 20% increase in sarcolemmal GLUT1 and a 60% increase in sarcolemmal GLUT4 contents in the ischemic region (P<0.05 for both). CONCLUSIONS: Insulin stimulates myocardial glucose utilization through translocation of GLUT1 as well as GLUT4. Insulin and ischemia have additive effects to increase in vivo glucose utilization and augment glucose transporter translocation. We conclude that recruitment of both GLUT1 and GLUT4 contributes to increased myocardial glucose uptake during moderate reductions in coronary blood flow under insulin-stimulated conditions.
Russell et al. (Tue,) conducted a other in Myocardial ischemia. Hyperinsulinemia and regional myocardial ischemia vs. Saline infusion and nonischemic regions was evaluated on Myocardial glucose uptake and sarcolemmal GLUT1 and GLUT4 content (p=<0.05). Hyperinsulinemia and regional myocardial ischemia additively increased myocardial glucose uptake by 50% and augmented sarcolemmal GLUT1 and GLUT4 contents by 20% and 60%, respectively (P<0.05).