Abstract Objective: Leptopus cordifolius Wall. ex Decne ( L . cordifolius ) is used in traditional medicine and has pharmacological properties including antioxidant and antidiabetic actions. However, little is known about the phytoconstituents of L . cordifolius that have antidiabetic properties. Materials and Methods: The crude methanolic extract (CME) obtained from the methanol extract of L . cordifolius was fractionated using column chromatography. The fractions with in vitro antioxidant and in vivo antidiabetic activity were characterized chemically using Fourier transform infrared spectrometer and nuclear magnetic resonance spectroscopies to identify the phytochemicals responsible for the antidiabetic activities. The antidiabetic activity of isolated fractions administered orally was assessed for 15 days at a dose of 100 (mg/kg) and glibenclamide (10 mg/kg). Results: The fraction crude methanolic extract fraction F (CMEF) was found to lower the fasting blood glucose level significantly ( P < 0.05) in diabetic mice and also inhibited significantly ( P < 0.05) both α-amylase and α-glucosidase enzymatic activities when compared with the remaining fractions. Chromatographic analysis of active fraction CMEF using various solvent systems resulted in four sub-fractions and four compounds. The compounds isolated were characterized and were confirmed to be sulfuretin, negletein, myricetin and kaemferol using spectral analysis. The flavonoid compounds isolated exhibited potent ( P < 0.05) hypoglycemic activities in mice which requires further validation. Conclusion: The results of this study indicate that the plant could be probed further for isolating some medically useful compounds.
Shahid et al. (Thu,) studied this question.
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