The aim of this study was to determine the dominant strains from samples of different hosts using molecular methods to contribute to the fight against cystic echinococcosis caused by Echinococcus granulosus, which is an important parasitic agent for human and animal health. Within the scope of this study, 75 cyst materials from humans, 25 cyst materials from cattle, and 25 cyst materials from sheep were used. After microscopic examination of cyst fluids, DNA was isolated from the cyst membrane/fluid and the NAD1 mitochondrial gene region was amplified by PCR. 60 cyst samples were analyzed by PCR amplicon sequence analysis and the strains of E. granulosus isolates were identified. Mutations in the NAD1 mitochondrial gene region were also identified. Hydatid cyst organ localization in sheep and cattle showed that liver localization was the most common in cattle while lung localization was the most common in sheep. When the results of sequence analysis were compared with the reference strains in NCBI, nine samples were found to be G3 (%15), and the remaining 51 (%85) samples were G1 strains. In this study, we determined, for the first time, that nine samples had mutations. The prevalence of G1 (sheep) strain in Van region indicates that sheep play an important role in the prevalence of E. granulosus in the region.
Halidi et al. (Thu,) studied this question.