Figure 4 from Generation, Transcriptomic States, and Clinical Relevance of CX3CR1+ CD8 T Cells in Melanoma

Antigen-specific CD8+ T cells CX3CR1− CD8+ T cells differentiate to CX3CR1+ subsets within the tumor. A, Experimental protocol. B16F10-bearing mice were either injected with ex vivo-activated Pmel-1 CD8+ T cells (WT) or Pmel-1 Cd2-cre/Cx3cr1DTR/DTR CD8+ T cells. The scheme of generation of Pmel-1 Cd2-cre/Cx3cr1DTR/DTR mice is shown in Supplementary Fig. S1. B, Representative contour plots showing CD27/DTR expression of CD8 and CD90.1-gated Cd2-cre/Cx3cr1DTR/DTR CD8+ T cells in the spleen with or without vaccination with hgp100 and CD40/TLR7 agonists in vivo. C, Representative contour plots of CD8 and CD90.1-gated cells in the spleen (top) and the tumor (bottom). Numbers, percentage of the CX3CR1+ subset. Data panels right show frequency of CX3CR1+ CD8+ T cells wild type (WT) or DTR-expressing Pmel-1 Cd2-cre/Cx3cr1DTR/DTR CD8+ T cells (DTR/DTR) obtained from four mice in each group. NS, not significant by two-tailed unpaired t test.