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Abstract Acute respiratory distress syndrome (ARDS) represents the most severe manifestation of acute hypoxic respiratory failure. Studies have shown that coagulation and fibrinolysis play an important role in the occurrence of ARDS. However, diagnostic biomarkers related to coagulation and fibrinolysis in ARDS have not been fully elucidated. In the GSE32707 dataset, the differences in gene expression levels between sepsis induced ARDS (SeARDS) group and sepsis group were compared, and 9 important genes related to hemolysis and fibrinolysis were identified. Subsequently, we used LASSO and Random Forest to screen these 9 genes and we identified 5 diagnostic markers that may participate in the progression of ARDS, including CCL4, ITIH4, TGM2, PI3 and F13A1. The nomogram built based on markers had good diagnostic efficiency. The same results appeared in the combined validation dataset. Finally, RT-qPCR analysis results revealed significant differences in the expressions of TGM2, PI3, and ITIH4 with sepsis and SeARDS patients. In conclusion, we identified 5 diagnostic markers that may be involved in the progression of ARDS, including CCL4, ITIH4, TGM2, PI3 and F13A1. Our analysis can provide a basis for a better understanding of the underlying molecular mechanisms of ARDS progression, thereby providing more reliable results for diagnosis.
Hao et al. (Fri,) studied this question.