Decoding Information Content underlying The Binary Codes of Nucleotide Bases of Codons

In the previous article 1, author had derived that each of the four nucleotide bases assumes a specific binary 2-bit code out of 24 choices available and this code is different for L-amino acids , D- Amino Acids and with respect to codons and anticodons. Moreover, the code was derived without considering the underlying physicochemical traits of the individual bases. In the current research article, physicochemical traits of bases are discussed and then matched with the binary bits of all the bases in the entire space of 24 configurations including the configurations pinned to the bases in extant genetic code. The Matching exercise reveals that for Standard Genetic Code, only specific 2 out 3 stated traits of the bases are tagged to the bits and that too in a specific order. Trait attached to the first bit cannot be interchanged with that of the second bit and vice versa. For the first time, a logical explanation is given. Moreover, the binary representation of bases is restricted to specific subset comprising of 4 configuration out of 24. Other novel and hitherto not discovered patterns emerge such as a new binary set of representation of bases comprising of 4 configurations which possibly manifests itself at transfer RNA level leading to possible different ways of information exchange. The analysis also leads, for the first time, to the existence of a 5th information molecule other than the current four bases (A, G, U/T, C) more as an explanation of anticodon binary representation than a de novo proposition.