FIGURE 8 from B7-H3–Targeting Chimeric Antigen Receptors Epstein-Barr Virus–specific T Cells Provides a Tumor Agnostic Off-The-Shelf Therapy Against B7-H3–positive Solid Tumors

B7H3.CAR EBVSTs targets MDSCs. A, Flow cytometric histograms of IL10, TGFβ, iNOS, and B7-H3 in MDSCs generated from 2 healthy donors (left). Median fluorescence intensities (MFI) of B7-H3 expressed on MDSCs from 2 healthy donors and the NCI-H1299 cell line (right). B, Cytotoxicity of UT or B7H3.CAR EBVSTs against allogeneic MDSCs. Data compiled from coculture of effector EBVSTs generated from 2 donors and target MDSCs generated from 2 other donors. C, Proliferation of UT and B7H3.CAR EBVSTs after anti-CD3/CD28 stimulation in the presence of allogeneic MDSCs. Each datapoint represents proliferation from each E:T donor pair. Proliferation index was calculated by normalizing percentages of proliferated cells against that of the no MDSC control condition. For B and C, UT versus B7H3.CAR EBVSTs groups were compared using Student unpaired t test. *, P P P