Study of gene expression of bacteria causing conjunctivitis

One hundred samples of patients with conjunctivitis who visited the Fallujah Teaching Hospital's eye consultation were included in the study. For both genders, the age range covered was 9 months to 77 years. The study found that 87% of the samples were positive for bacterial growth, while 13% were negative for it. 56.32% of them were for males and 43.67% for females. A variety of bacteria were isolated, including Staphylococcus aureus, Staph-epidermidis, and E. coli. The study demonstrates that the minimum inhibitory concentration (MIC) of each black tea aqueous extract and vancomycin was 12.5, which gives 100% inhibition. The inhibition effects of synergistic black tea extract and vancomycin were at concentrations of 6.2 giving 100% inhibition. Also, black tea extract has a beneficial effect on the gene expression of the 16S rRNA gene of E. coli bacteria in cooperation with untreated samples, which is down-regulation. Furthermore, the black tea extract affected all types of Staph-epidermidis bacteria by increasing their gene expression (upregulation) in cooperation with the untreated sample