Key points are not available for this paper at this time.
Abstract Background We evaluated whether the sputum bacterial microbiome differs between nontuberculous mycobacteria pulmonary disease (NTM-PD) patients with stable disease not requiring antibiotic treatment and those requiring antibiotics. Methods We collected sputum samples from 21 clinically stable NTM-PD patients (stable group) and 14 NTM-PD patients needing antibiotic treatment (treatment group). We also obtained 13 follow-up samples from the stable group. We analyzed the 48 samples using 16S rRNA gene sequencing (V3–V4 region) and compared the groups. Results In the linear discriminant analysis effect size (LEfSe) analysis, the species Porphyromonas pasteri , Haemophilus parahaemolyticus , Prevotella nanceiensis , and Gemella haemolysans were significantly more prevalent in the sputum of the stable group compared to the treatment group. No taxa showed significant differences in alpha-/beta-diversity or LEfSe between the 21 baseline and 13 follow-up sputum samples in the stable group. In the stable group, the genus Bergeyella and species Prevotella oris were less common in patients who achieved spontaneous culture conversion ( n = 9) compared to those with persistent NTM positivity ( n = 12) (effect size 3.04, p = 0.039 for Bergeyella ; effect size 3.64, p = 0.033 for P . oris ). In the treatment group, H . parainfluenzae was more common in patients with treatment success ( n = 7) than in treatment-refractory patients ( n = 7) (effect size 4.74, p = 0.013). Conclusions Our study identified distinct bacterial taxa in the sputum of NTM-PD patients based on disease status. These results suggest the presence of a microbial environment that helps maintain disease stability.
Song et al. (Sat,) studied this question.