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Abstract ID 97991 Poster Board 469 Background: Aging increases risk for the development of vascular cognitive impairment. Impaired bioenergetics, mitochondrial oxidative phosphorylation (OxPhos) and glycolysis, in the brain microvascular endothelium induce neurovascular uncoupling, which is one of the underlying mechanisms of vascular cognitive decline. Bone marrow-derived adult CD34+ stem/progenitor cells are mobilized into the blood stream in physiological conditions and maintain endothelial health and regeneration via paracrine angiogenic mechanisms largely mediated by exosomes. Aging is associated with impaired endothelial regeneration and vascular repair by CD34+ stem cells due to paracrine dysfunctions. This study tested the hypothesis that CD34+ stem cell-derived exosomes (CD34-Exo) of older adults impair bioenergetics in the human brain microvascular endothelial cells (hBMVECs). Methods: CD34+ cells were isolated from peripheral blood mononuclear cells derived from subjects, 18 - 35 (Young) or > 60 (Old) years of age. Exosomes were collected from the cell-supernatants by ultracentrifugation. The size distribution of exosomes was analyzed by using a Nanosight and exosome-imaging was carried out by using an Atomic force microscope. Cultured hBMVECs (passage 6 or 7) were exposed to exosomes and angiogenesis was evaluated by carrying out Matrigel assay. Agilent Seahorse bioanalyzer was used to determine OxPhos and glycolysis. 'n' represents the number of subjects the CD34-Exos were derived from. Results: Old CD34-Exos impaired angiogenesis in hBMVECs (decreased tube length and the number branches and branch points, P P P Conclusion: The study infers that Old CD34-Exos impair mitochondrial and glycolytic metabolism, and angiogenesis in hBMVECs, which collectively contribute to vascular cognitive decline in aging. This study is supported by the National Institute of Aging of National Institutes of Health, AG056881.
Jarajapu et al. (Mon,) studied this question.