FIGURE 1 from Characterizing Neutrophil Subtypes in Cancer Using scRNA Sequencing Demonstrates the Importance of IL1β/CXCR2 Axis in Generation of Metastasis-specific Neutrophils

Characterization of neutrophil signatures and lineages in health and PTs. A, Uniform Manifold Approximation and Projection for Dimension (UMAP) plot of healthy neutrophils grouped by tissue type. BM: healthy bone marrow, SP: healthy spleen, L: healthy lung, BL: healthy blood. B, UMAP plots of healthy and tumor-derived neutrophils grouped by tissue type and Seurat clusters (0–15). BM: healthy bone marrow, SP: healthy spleen, L: healthy lung, BL: healthy blood, LAC: lung adenocarcinoma, KPN: colorectal cancer with Kras, Trp53 and Notch mutations. C, UMAP plot of neutrophils in mouse breast cancer model. WT: healthy breast tissue, PyMT: polyomavirus middle-T oncoprotein tumor. D and E, Scoring of Hₑnriched and Tₑnriched neutrophil signatures. F, UMAP plot of neutrophils in mouse colorectal cancer model. All neutrophils are tumor-derived. G and H, Hₑnriched and Tₑnriched signatures in colorectal cancer (CRC). I, UMAP plot of human NSCLC neutrophils. Blood: blood-derived, tumor: tumor-derived. J and K, Hₑnriched and Tₑnriched signatures NSCLC. L, UMAP plot of human breast carcinoma (BC) neutrophils. Most neutrophils are tumor-derived. M and N, Hₑnriched and Tₑnriched signatures in breast carcinoma. O–R, Unsupervised pseudotime analysis of neutrophils in mouse and human datasets. Lineages in the individual datasets are numbered. S–V, Il1β/IL1β is differentially expressed at the end of tumor-specific lineages. W–Z, Estimated smoothers for Il1β/IL1β expression over pseudotime across the different lineages.