Global aquaculture expansion necessitates sustainable technologies like biofloc technology (BFT), and the type of carbon source exerts a crucial impact on the functionality of biofloc systems. This study aimed to evaluate the effects of single carbon sources (glucose G and maltodextrin M) and their 1:1 mixture (GM) on water quality, microbial communities, growth performance, and physiological status of redclaw crayfish ( Cherax quadricarinatus ) in BFT systems, with a clear‐water group (F) as the control. A 70‐day experiment was conducted in triplicate tanks per treatment, with key parameters (water quality indices, microbial diversity via 16S rRNA sequencing, growth metrics, and hepatopancreatic antioxidant enzyme activities) measured. Data were analyzed using one‐way ANOVA followed by Tukey’s Honestly Significant Difference (HSD) test ( p 1.40). This research provides a practical microbial‐based strategy for improving redclaw crayfish culture and crustacean aquaculture sustainability via optimized carbon source management in BFT.
Ruan et al. (Thu,) studied this question.