Abstract 3D printing is a growing technique that allows building complex structures with controlled properties. Scaffolds are essential as support for cells, being commonly produced using hydrogels. In this work, the printing parameters for agarose were analyzed and optimized, observing that agarose is a printable biomaterial. The best accuracy, considering a line, was 97.29%, for 2% of agarose and using a 25G needle at 6 mm/s and 40 kPa. Moreover, the shape fidelity of a grid was improved by adding 3% of laponite, obtaining an accuracy over 83%. Afterwards, a scaffold was successfully printed, which can be further investigated for tissue engineering applications. Graphical Abstract
Teixeira et al. (Fri,) studied this question.