Cyanidin-3-glucoside confers neuroprotection in ischemic stroke by targeting NOX4-mediated oxidative stress: A network pharmacology and experimental validation study

Key Points

• Investigate how cyanidin-3-glucoside confers neuroprotection in ischemic stroke via oxidative stress modulation.
• Used network pharmacology to identify targets of cyanidin-3-glucoside.
• Conducted experimental validation of findings using ischemia-reperfusion models.
• Analyzed the Nrf2-Keap1-HO-1 antioxidant pathway related to Nox4.
• Cyanidin-3-glucoside reduced oxidative stress in ischemia-reperfusion injury.
• Targeting Nox4 was significant in mitigating tissue damage.
• Modulation of the Nrf2-Keap1-HO-1 pathway supported its neuroprotective effects.