An integrative strategy for comprehensive determination of nineteen constituents from Eupatorii Herba and application in pharmacokinetic study by combing UHPLC-QTRAP-MS/MS with gC-MS

Key Points

• This research aims to develop a comprehensive method for quantifying multiple constituents from Eupatorii Herba and to study their pharmacokinetics.
• Combined UHPLC-QTRAP-MS/MS with GC-MS for analysis.
• Validated method assessed for precision, accuracy, recovery, matrix effect, and stability.
• Analytes quantified in rat plasma samples.
• Validated method showed precision, accuracy, and robust recovery rates.
• Rapid absorption detected for all analytes in plasma.
• Melilotoside, o-acetyl-p-cresol, and thymol had the highest systemic exposure.