Brown adipose tissue transplantation prevented the development of impaired glucose tolerance after myocardial infarction in high-fat-fed mice.
RCT
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No
Does brown adipose tissue (BAT) transplantation prevent glucose intolerance and cardiac remodeling in high-fat-fed mice after a mild myocardial infarction?
Increasing brown adipose tissue mass via transplantation protects against adverse cardiac remodeling, exercise intolerance, and impaired glucose metabolism following a mild myocardial infarction in an obese mouse model.
Effect estimate: null (95% CI null)
p-value: p=null
Abstract Background Obesity increases the risk of developing impaired glucose tolerance (IGT) and type 2 diabetes (T2D) after myocardial infarction (MI). Brown adipose tissue (BAT) is important to combat obesity and T2D, and increasing BAT mass by transplantation improves glucose metabolism and cardiac function. The objective of this study was to determine if BAT had a protective effect on glucose tolerance and cardiac function in high-fat diet (HFD) fed mice subjected to a mild MI. Methods Male C57BL/6 mice were fed a HFD for eight weeks and then divided into Sham (Sham-operated) and +BAT (mice receiving 0.1 g BAT into their visceral cavity). Sixteen weeks post-transplantation, mice were further subdivided into ±MI (Sham; Sham-MI; +BAT; +BAT-MI) and maintained on a HFD. Cardiac (echocardiography) and metabolic function (glucose and insulin tolerance tests, body composition and exercise tolerance) were assessed throughout 22 weeks post-MI. Quantitative PCR (qPCR) was performed to determine the expression of genes related to metabolic function of perigonadal adipose tissue (pgWAT), subcutaneous white adipose tissue (scWAT), liver, heart, tibialis anterior skeletal muscle (TA); and BAT. Results +BAT prevented the increase in left ventricle mass (LVM) and exercise intolerance in response to MI. Similar to what is observed in humans, Sham-MI mice developed IGT post-MI, but this was negated in +BAT-MI mice. IGT was independent of changes in body composition. Genes involved in inflammation, insulin resistance, and metabolism were significantly altered in pgWAT, scWAT, and liver in Sham-MI mice compared to all other groups. Conclusions BAT transplantation prevents IGT, the increase in LVM, and exercise intolerance following MI. MI alters the expression of several metabolic-related genes in WAT and liver in Sham-MI mice, suggesting that these tissues may contribute to the impaired metabolic response. Increasing BAT may be an important intervention to prevent the development of IGT or T2D and cardiac remodeling in obese patients post-MI.
Silva et al. (Fri,) conducted a rct in Impaired glucose tolerance and cardiac remodeling post-myocardial infarction. Brown adipose tissue transplantation vs. Sham (no BAT transplantation) was evaluated on Impaired glucose tolerance (null, 95% CI null, p=null). Brown adipose tissue transplantation prevented the development of impaired glucose tolerance after myocardial infarction in high-fat-fed mice.