What question did this study set out to answer?

The aim is to molecularly characterize the genus Bychowskyella and differentiate its species using 18S rDNA/rRNA.

February 14, 2026Open Access

Molecular and Phylogenetic Analysis of Genus Bychowskyella, using the 18S rDNA/18S rRNA for Studying Conserveness in the Genome for Species- Level Differentiations and Uses for the Taxonomy

Key Points

The aim is to molecularly characterize the genus Bychowskyella and differentiate its species using 18S rDNA/rRNA.
Performed molecular characterization using 18S rDNA/rRNA sequences
Conducted phylogenetic analysis using Neighbor Joining and Maximum Parsimony methods
Utilized MEGA software for phylogenetic tree construction
Calculated GC content using Vectorbuilder tool
Analyzed secondary structure conserveness with ExpaRNA and motif predictions with MEME Suite
Successfully differentiated species within Bychowskyella at the molecular level
Phylogenetic trees indicated significant genetic variations among species
Identified conserved regions in secondary structures, enhancing understanding of the genome
Motif predictions confirmed molecular-based findings

Abstract

Abstract: The Current Research Deals with the Molecular Characterization of Genus Bychowskyella and Comparison among its Species based on 18S rDNA/rRNA, as earlier studies were only focused on Morphological and Morphometric Features which fails to differentiate accurately the closely related species. Phylogenetic Studies have been made based on Construction of Neighbour Joining and Maximum Parsimony Methods, using the MEGA Software. Vectorbuilder tool used for the calculation of Percentage of GC Content in the Genome. Using the ExpaRNA tool Conserveness in the loops of Secondary Structure are made, and MEME Suite Software is used Lastly for Motif Predictions and Confirmation of the Molecular based Studies. This Study further opens the door for many future Researches in the Areas of Parasitology, which will help in combating many Unknown Diseases possibly linked to the Genus Bychowskyella, Studying Host Parasite Interactions. Thus the entire Research bridges the gap between Morphological Based Studies and Molecular Based Studies, Helping in Accurate and Reliable way to differentiate between the Species within the Genus Bychowskyella with that of other Genus in Monogenea. Keywords: Genus Bychowskyella, Monogenea, 18S rDNA/rRNA, Phylogenetic Analysis, Secondary Structure, Motif Predictions. Title: Molecular and Phylogenetic Analysis of Genus Bychowskyella, using the 18S rDNA/18S rRNA for Studying Conserveness in the Genome for Species- Level Differentiations and Uses for the Taxonomy Author: Sammohan Sameeran Pati, Haren Ram Chiary, Pukhrambam Pushpa Devi, Amit Singh Dhaulaniya International Journal of Life Sciences Research ISSN 2348-313X (Print), ISSN 2348-3148 (online) Vol. 14, Issue 1, January 2026 - March 2026 Page No: 7-12 Research Publish Journals Website: www.researchpublish.com Published Date: 09-February-2026 DOI: https://doi.org/10.5281/zenodo.18549709 Paper Download Link (Source) https://www.researchpublish.com/papers/molecular-and-phylogenetic-analysis-of-genus-bychowskyella-using-the-18s-rdna18s-rrna-for-studying-conserveness-in-the-genome-for-species--level-differentiations-and-uses-for-the-taxonomy

Molecular and Phylogenetic Analysis of Genus Bychowskyella, using the 18S rDNA/18S rRNA for Studying Conserveness in the Genome for Species- Level Differentiations and Uses for the Taxonomy

Key Points

Abstract

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