In vitro cultured human-derived HFMSCs have shown the potential to promote wound

Effective wound healing is vital for tissue repair, and human hair follicle-derived mesenchymal stem cells (HFMSCs) have emerged as a promising therapeutic candidate for enhancing wound repair due to their inherent regenerative capabilities. We established an in vitro culture system for HFMSCs and confirmed their mesenchymal identity through flow cytometry and trilineage differentiation potential. Leveraging single-cell RNA sequencing (scRNA-seq), we conducted a comparative transcriptomic analysis between HFMSCs and umbilical cord-derived MSCs (UCMSCs), revealing distinct molecular signatures. HFMSCs exhibited pronounced enrichment in extracellular matrix (ECM) remodeling pathways (e.g., collagen synthesis, integrin signaling) and progenitor cell markers (CSPG4, NES), while UCMSCs showed enriched expression profiles in cell proliferation. Functionally, HFMSCs demonstrated superior migratory capacity in scratch assays and accelerated wound closure in a murine full-thickness skin defect model. Our findings highlight the therapeutic potential of HFMSCs in wound healing and provide insights into their underlying molecular mechanisms, suggesting a promising approach for advanced wound healing therapies