What question did this study set out to answer?

The study aims to evaluate the virulence and pathogenesis of Aeromonas infection using a C. elegans model.

February 28, 2026

Muscle Necrosis Assay in C. elegans Induced by a Pathogenic Aeromonas Strain

Key Points

The study aims to evaluate the virulence and pathogenesis of Aeromonas infection using a C. elegans model.
Utilized GFP-expressing C. elegans to assess muscle necrosis.
Infected worms were fed on Aeromonas-seeded plates.
Monitored tissue damage through fluorescence imaging.
Observed progressive loss of muscle structure in infected worms.
Noted decrease in GFP signal correlating with muscle necrosis.
Confirmed Aeromonas strain induces a necrotic response in C. elegans.

Abstract

Source: Chen, Y. et. al., Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model. J. Vis. Exp. (2018)This video demonstrates a muscle necrosis assay in C. elegans (Caenorhabditis elegans) using pathogenic Aeromonas strains. By feeding green fluorescent protein (GFP)-expressing worms on Aeromonas-seeded plates, the assay models bacterial infection and reveals tissue damage through fluorescence imaging. The progressive loss of muscle structure and GFP signal indicates an Aeromonas-induced necrotic response.

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