Our assay allows specific quantification of β2-integrin-mediated affinity and avidity of T cells in whole blood samples. In congruence to human adhesion, these mechanisms are ROS and Ca2+ dependent and significantly elevated after myocardial infarction. Our refined and robust assay may be of particular use in phenotyping involved mechanisms in T cell activation in atherosclerotic cardiovascular disease.
Therre et al. (Wed,) studied this question.