Clec12a is an immunoregulatory C-type lectin receptor expressed on dendritic cells as well as other myeloid cells, and involved in maintaining immune homeostasis. Unlike many other C-type lectin receptors that promote immune activation, Clec12a transmits inhibitory signals through an immunoreceptor tyrosine-based inhibitory motif, thereby contributing to immune regulation. Although the extracellular domain of murine Clec12a has been produced in previous studies, its suitability as a functional bait for directed evolution–based ligand discovery has not been established, nor have quantitative yield and scalability been reported. Here, we established a mammalian expression system that reproducibly yields ∼80 mg/L of the mClec12a extracellular domain, providing material of sufficient quality and quantity for directed evolution of peptide binders. Incorporation of an interleukin-2 signal peptide enabled efficient secretion from Expi293 cells, and the purified protein retained N- glycosylation, indicating proper maturation through the endoplasmic reticulum-Golgi pathway. Using ribosome display with mClec12a as bait, three mClec12a binding peptides (CBP1-3) were isolated, with CBP2 showing the highest affinity ( K D ≈ 6.29 μM). Importantly, CBP2 bound to primary dendritic cells derived from mouse spleen and bone marrow, supporting the native-like conformation and functional integrity of the recombinant mClec12a used for selection. The identified peptide represents a first-generation mClec12a + cell targeting ligand and provides a foundation for further optimization toward dendritic cell targeting and immunomodulatory applications. • The murine Clec12a was expressed in mammalian cells in a yield of ∼80 mg/L. • The purified protein underwent N -glycosylation as a post-translational modification. • Recombinant Clec12a served as a native-like bait for ribosome display-based peptide screening. • Selected peptides bound to Clec12a-expressing primary dendritic cells. • The results indicated that the native folding of recombinant Clec12a was maintained.
Kawaguchi et al. (Sat,) studied this question.