Application of a New UPLC–MS/MS Method for the Analysis of Salicylic Acid and Its Metabolites, Gentisic Acid and Salicyluric Acid, in Rat Serum and Urine

Key Points

• Salicylic acid and its metabolites were effectively analyzed in rat serum and urine samples, demonstrating the method's effectiveness.
• The new UPLC-MS/MS method provided accurate quantification of gentisic acid and salicyluric acid alongside salicylic acid.
• Our observational analysis highlighted the method's sensitivity and reliability across various biological samples.
• These findings support advanced metabolite profiling in preclinical models; validation across different species is needed.