Synergistic effects of H2O2, melatonin, and L-cysteine on biochemical traits and alkaloid accumulation in Catharanthus roseus L. “Ocellatus” in vitro plants

Key Points

• Increased alkaloid accumulation resulted from the synergy of H2O2, melatonin, and L-cysteine in plants.
• Notable enhancement in biochemical traits was observed with the combined effects of the three compounds studied.
• Analysis based on in vitro assessment of Catharanthus roseus plants shows significant treatment interactions.
• Findings suggest potential for improved alkaloid production, highlighting relevance in medicinal plant cultivation.