Digital spatial profiling of α-PD-1 treated breast cancer bone metastases reveals region-specific signaling and enrichment of immune-suppressive markers

Key Points

• Bone metastases exhibited unique signaling patterns distinct from primary tumors, indicating specialized tumor microenvironments.
• Significant enrichment of immune-suppressive markers was found in specific regions of the breast cancer lesions.
• Digital spatial profiling techniques were employed to map protein expression and cellular interactions in samples from breast cancer cases.
• Findings may highlight targets for therapeutic strategies in managing breast cancer bone metastases, although further functional studies are needed.