March 3, 2026

Identifikace genů a proteinů, regulovaných Ser/Thr proteinkinasou Streptococcus pneumoniae, mikročipovou metodou a analýzou 2-D gelů

Key Points

The transcription profile shows significant upregulation of 49 genes in the DstkP strain, indicating the kinase's role in regulation.
Out of 2,300 assessed genes, 48 exhibited a downregulation, highlighting a complex interaction of gene expression.
Analysis utilized dna microarray technology followed by proteomic studies to validate findings through mass spectrometry.
These results suggest potential target loci for understanding Streptococcus pneumoniae's pathogenic mechanisms.

Abstract

To identify the genes whose expression is controlled by protein kinase StkP we examined the transcription profile of DstkP mutant by DNA microarray technology. The transcript analysis revealed the StkP-dependent expression of many potential target loci. Out of 2,300 genes evaluated, 49 were found to be specifically and strongly upregulated in the DstkP strain, whereas 48 were downregulated. The data obtained from transcription profile were further extended by proteomic studies and mass spectrometry.

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Identifikace genů a proteinů, regulovaných Ser/Thr proteinkinasou Streptococcus pneumoniae, mikročipovou metodou a analýzou 2-D gelů

Key Points

Abstract

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