Discovery and engineering of ferulic acid esterase AmCE1 for aromatic acyl transfer in aqueous phase

Key Points

• Enhanced transfer of aromatic acyl groups is achieved by engineered ferulic acid esterase AmCE1, demonstrating significant biocatalytic potential.
• The engineering led to a notable increase in activity, as seen in comparative assays with traditional methods.
• Evaluation employed an innovative approach by using aqueous systems to measure enzyme activity in real-time.
• This innovation may enable new methods for producing aromatic compounds, highlighting the importance of enzyme engineering for industrial applications.