Isolation of Mouse Bladder Epithelial Cells Containing Intracellular Bacterial Communities

Key Points

• Single intracellular bacterial communities were isolated from mouse bladder epithelials, highlighting their presence during urinary infections.
• Manual micropipetting facilitated the isolation of epithelial cells, allowing for detailed downstream analysis of E. coli.
• E. coli populations associated with urinary tract infections were analyzed at the single-cell level, revealing their viability and behavior.
• The study calls for further exploration of bacterial communities in urinary models, as they could inform treatment strategies for infections.