Engineering aptasensor based on DNAzyme-aptamer switch and loop-mediated isothermal amplification for ultrasensitive contaminant detection

Key Points

• The aptasensor enables ultrasensitive detection of contaminants—improving diagnostic accuracy and speed.
• Its performance was validated with a detection limit in the low nanomolar range, showcasing significant sensitivity.
• Analysis employs loop-mediated isothermal amplification for rapid, efficient biomarker detection under controlled conditions.
• This novel engineering approach may enhance contamination monitoring in various environments and applications.