Comparative performance of standalone E6/E7 mRNA testing versus a combined DNA genotyping and viral load assay for detecting cervical high-grade lesions: a retrospective paired study

Key Points

• The E6/E7 mRNA assay provides higher specificity and positive predictive value for detecting CIN2+ lesions, enhancing precision screening for cervical cancer.
• Results show the E6/E7 mRNA assay has improved specificity without significant loss in sensitivity compared to a DNA-based strategy, indicating its robustness in screenings.
• Analysis of retrospective paired data highlights the effective performance of the E6/E7 mRNA assay in young women and those with ASCUS and HPV DNA positivity.
• The findings support the continued use of E6/E7 mRNA assays for cervical cancer screening, particularly in reducing unnecessary follow-ups for lower-grade lesions.