Multiplex edited universal CAR T cells without the genomic cost

Key Points

• The aim is to develop CAR T cells that are universally applicable and avoid the risks associated with genomic modifications.
• Utilized multiplex editing techniques to modify CAR T cells.
• Focused on creating universal CAR T cells that can target multiple antigens.
• Assessed the immune response of edited cells in vitro and in vivo.
• Multiplex edited CAR T cells showed improved activation in response to target antigens.
• No adverse genomic changes were detected post-editing.
• The universal CAR T cells demonstrated effective tumor targeting in preliminary models.