Comparative Effectiveness of Soya Bean Lecithin and Liposome Based Semen Extenders in Cryopreservation of Boer and Anglo-Nubian Goat Semen

This study investigated the effects of two commercially available non-animal-derived extenders with different mechanisms of action on post-thaw spermatological characteristics in Boer and Anglo-Nubian goat breeds during the non-breeding season. Ejaculates were collected weekly from ten goats (Boer, n=5; Anglo-Nubian, n=5) over six replicates, with individual cryopreservation performed for each sample. Each ejaculate was divided into two aliquots: one diluted with soy lecithin-based AndroMed and the other with liposome-based OptiXcell prior to cryopreservation. Post-thaw assessment included Computer-Assisted Sperm Analysis (CASA) for total and progressive motility (%) and kinematic parameters, while flow cytometry evaluated plasma membrane integrity, acrosome integrity, mitochondrial membrane potential, and quantified superoxide radicals to determine oxidative stress status. Results revealed that Anglo-Nubian samples exhibited comparable CASA and flow cytometry parameters between extenders (p0.05). However, Boer samples demonstrated significantly higher total and progressive motility, as well as elevated VAP, VSL, VCL, ALH, and BCF values in the AndroMed-diluted group (p0.05). Additionally, oxidative stress levels were significantly lower in Boer semen extended with AndroMed compared to OptiXcell (p0.05). These results indicate breed-dependent extender efficacy, with AndroMed exhibiting superior performance in Boer goats while both extenders yielded equivalent outcomes in Anglo-Nubian goats. This breed-specific response pattern provides comparative evidence of differential breed responses to soy lecithin- and liposome-based extenders, directly supporting the need for breed-tailored semen preservation strategies. Consequently, these findings demonstrate that extender selection critically determines post-thaw sperm quality, confirming breed-specific compatibility as an essential criterion for optimal semen cryopreservation protocols.