The electro-oxidation of eugenol (EUG) natural antioxidant was studied by cyclic voltammetry in phosphate buffer solutions (PBS) of different pH at electrochemically partially reduced graphene oxide (GCE/ePRGO). The voltammetric responses were mainly controlled by adsorption at this modified electrode. Current values were higher at pH 2.0 PBS, therefore, this pH was chosen to perform all experiments. DFT calculations of pKa’s and standard potentials defined the possible pathways of eugenol and its oxidation products. These pathways were evaluated through the comparison of voltammetric simulations of adsorbed species with experiments at pH 2.0, which also allowed for the estimation of the values of the kinetic parameters involved in electrochemistry. Our findings suggest a multi-step redox process in which Eugenol is first oxidized to the radical species and then to a cationic product. At this stage, the pathways branch into to methylenquinone and a 4-allyl-1,2-diquinone molecules. 4-allyl-1,2-diquinone is finally reduced in single or double reversible electrochemical step to the hydroquinone species. The present physicochemical work allows for a deeper understanding of the eugenol oxidation mechanism, which was only partially proposed in previous studies.
Pierini et al. (Sat,) studied this question.